Table III.
Top 75 protein list of CB nLC-ESI-MS/MS and nLC-MALDI-MS/MS experiments
Shown are the number of matched ESI and MALDI peptides, accession number, mass, length, pI, and Mascot hit numbers. “None” indicates that the protein was absent from the respective experiment. Proteins with predicted TM stretches are highlighted by bold type. For a complete list of all proteins see supplemental Table S2. ABC, ATP-binding cassette; PTS, phosphotransferase system.
| No. | Peptides found
|
Accession no. | Protein description | Mascot hits
|
Protein mass (Da) | Protein length | Protein pI | ||
|---|---|---|---|---|---|---|---|---|---|
| ESI | MALDI | ESI | MALDI | ||||||
| 1 | 97 | 166 | P00772 | Elastase 1 | 2 | 1 | 28,803 | 266 | 8.42 |
| 2 | 144 | 61 | Cg1537 | ptsG; glucose-specific enzyme II BC component of PTS | 1 | 3 | 72,525 | 683 | 5.13 |
| 3 | 74 | 72 | Cg0446 | sdhA; succinate dehydrogenase A | 3 | 2 | 74,632 | 673 | 5.37 |
| 4 | 71 | 46 | Cg2705 | amyE; maltose-binding protein precursor | 4 | 4 | 49,417 | 443 | 4.36 |
| 5 | 65 | 22 | Cg3009 | Hypothetical protein predicted by Glimmer/Critica | 5 | 9 | 6,158 | 57 | 3.47 |
| 6 | 62 | 10 | Cg2181 | ABC-type peptide transport system, secreted component | 6 | 23 | 57,653 | 534 | 3.9 |
| 7 | 47 | 3 | Cg2120 | ptsF; sugar specific PTS system, fructose mannitol-specific transport protein | 7 | 63 | 70,457 | 688 | 5.31 |
| 8 | 39 | 47 | Cg2409 | ctaC; cytochrome c oxidase chain II | 10 | 5 | 39,607 | 359 | 4.74 |
| 9 | 8 | 46 | Cg0587 | tuf; elongation factor Tu | 47 | 6 | 43,824 | 396 | 4.92 |
| 10 | 44 | 22 | Cg2911 | ABC-type manganese/zinc transport system, secreted manganese/zinc-binding (lipo)protein | 8 | 8 | 33,986 | 314 | 4.09 |
| 11 | 41 | 22 | Cg0447 | sdhB; succinate dehydrogenase B | 11 | 10 | 26,631 | 249 | 5.4 |
| 12 | 39 | 2 | Cg2925 | ptsS; enzyme II sucrose protein | 9 | 181 | 69,104 | 661 | 4.96 |
| 13 | 30 | 17 | Cg2404 | qcrA1; Rieske iron-sulfur protein | 16 | 17 | 45,156 | 408 | 5.29 |
| 14 | 29 | 9 | Cg1366 | atpA; probable ATP synthase α chain protein | 14 | 38 | 58,715 | 547 | 4.86 |
| 15 | 28 | 26 | Cg3138 | Membrane protease subunit, stomatin/prohibitin homolog | 13 | 7 | 34,765 | 325 | 4.77 |
| 16 | 27 | 21 | Cg2780 | ctaD; probable cytochrome c oxidase polypeptide subunit | 15 | 12 | 65,059 | 584 | 6.91 |
| 17 | 27 | 19 | Cg2403 | qcrB; cytochrome b, membrane protein | 19 | 15 | 59,770 | 539 | 6.38 |
| 18 | 26 | 13 | Cg2708 | msiK1; ABC-type sugar transport system, ATPase component | 12 | 18 | 40,290 | 376 | 5.53 |
| 19 | 25 | 12 | Cg2875 | Hypothetical protein predicted by Glimmer | 17 | 22 | 3,872 | 38 | 3.92 |
| 20 | 23 | 0 | Cg1332 | Putative secreted hydrolase | 21 | None | 55,920 | 527 | 4.4 |
| 21 | 22 | 1 | Cg3182 | cop1; trehalose corynomycoyl transferase | 18 | 148 | 70,616 | 657 | 4.85 |
| 22 | 22 | 5 | Cg1363 | atpE; ATP synthase C chain | 23 | 76 | 8,117 | 80 | 4.42 |
| 23 | 22 | 1 | Cg0413 | cmt1; trehalose corynomycoyl transferase | 27 | 426 | 39,481 | 365 | 4.56 |
| 24 | 19 | 2 | Cg0683 | Permease | 24 | 153 | 55,630 | 533 | 5.78 |
| 25 | 18 | 6 | Cg0953 | Na+/proline, Na+/pantothenate symporter | 20 | 47 | 57,232 | 551 | 5.98 |
| 26 | 18 | 5 | Cg0737 | ABC-type transport system, secreted lipoprotein component | 30 | 49 | 31,722 | 299 | 4.05 |
| 27 | 16 | 18 | Cg1656 | ndh; NADH dehydrogenase | 22 | 13 | 50,926 | 467 | 5.68 |
| 28 | 17 | 11 | Cg1368 | atpD; ATP synthase α subunit | 31 | 20 | 52,519 | 483 | 4.74 |
| 29 | 12 | 17 | Cg2843 | pstB; ABC-type phosphate transport system, ATPase component | 33 | 16 | 28,196 | 257 | 5.71 |
| 30 | 4 | 17 | Cg0583 | fusA; elongation factor G | 105 | 11 | 77,842 | 709 | 4.81 |
| 31 | 16 | 5 | Cg3008 | porA; porin | 38 | 94 | 4,677 | 45 | 3.77 |
| 32 | 15 | 2 | Cg1603 | Conserved membrane protein | 25 | 88 | 41,873 | 397 | 4.91 |
| 33 | 15 | 9 | Cg3186 | cmt2; trehalose corynomycoyl transferase | 26 | 26 | 37,163 | 341 | 5.41 |
| 34 | 15 | 6 | Cg3365 | rmpC; putative ribitol transport membrane protein | 28 | 62 | 52,700 | 513 | 8.78 |
| 35 | 15 | 9 | Cg3186 | cmt2; trehalose corynomycoyl transferase | 26 | 26 | 37,163 | 341 | 5.41 |
| 36 | 14 | 1 | Cg1314 | putP; proline transport system | 35 | 103 | 56,610 | 524 | 4.91 |
| 37 | 14 | 4 | Cg0834 | Bacterial extracellular solute-binding protein | 39 | 81 | 45,049 | 424 | 3.85 |
| 38 | 2 | 14 | Cg0957 | fas-IB; fatty-acid synthase | 32 | 14 | 314,960 | 2969 | 4.74 |
| 39 | 13 | 7 | Cg1081 | ABC-type multidrug transport system, ATPase component | 29 | 24 | 33,285 | 308 | 4.77 |
| 40 | 13 | 5 | Cg1556 | Conserved hypothetical protein | 44 | 65 | 33,325 | 304 | 9.68 |
| 41 | 13 | 4 | Cg1362 | atpB; ATP synthase F0 subunit 6 | 78 | 92 | 36,385 | 322 | 9.51 |
| 42 | 12 | 10 | Cg0924 | ABC-type cobalamin/Fe3+-siderophores transport system | 34 | 28 | 35,668 | 338 | 4 |
| 43 | 12 | 9 | Cg0756 | cstA; putative carbon starvation protein A | 37 | 33 | 81,038 | 759 | 6.06 |
| 44 | 12 | 1 | Cg1082 | Putative membrane protein | 41 | 182 | 30,196 | 277 | 9.26 |
| 45 | 12 | 1 | Cg0359 | Putative membrane protein | 64 | 1057 | 21,555 | 201 | 9.62 |
| 46 | 1 | 12 | Cg1787 | ppc; phosphoenolpyruvate carboxylase | 295 | 19 | 103,134 | 919 | 4.92 |
| 47 | 0 | 12 | Cg0791 | pyc; pyruvate carboxylase | None | 21 | 123,027 | 1140 | 5.39 |
| 48 | 11 | 1 | Cg2262 | ftsY; signal recognition particle GTPase | 52 | 173 | 60,607 | 576 | 4.33 |
| 49 | 11 | 1 | Cg2361 | Cell division initiation protein, antigen 84 homolog | 61 | 348 | 38,662 | 365 | 4.87 |
| 50 | 10 | 11 | Cg0445 | sdhCD; succinate dehydrogenase CD | 85 | 27 | 28,354 | 257 | 9.77 |
| 51 | 10 | 0 | Cg2810 | Na+/H+-Dicarboxylate symporter | 36 | None | 47,955 | 465 | 7.71 |
| 52 | 10 | 4 | Cg2195 | Putative secreted or membrane protein | 43 | 68 | 11,897 | 115 | 4.1 |
| 53 | 10 | 0 | Cg3368 | ABC transporter permease protein | 53 | None | 57,131 | 528 | 9.02 |
| 54 | 10 | 0 | Cg2912 | ABC-type cobalamin/Fe3+-siderophores transport system | 54 | None | 24,475 | 230 | 9.34 |
| 55 | 6 | 10 | Cg2196 | Putative secreted or membrane protein | 153 | 29 | 12,721 | 119 | 4.21 |
| 56 | 9 | 5 | Cg2845 | pstC; ABC-type phosphate transport system, permease component | 42 | 59 | 37,569 | 355 | 9.66 |
| 57 | 9 | 4 | Cg2704 | ABC-type sugar transport system, permease component | 63 | 75 | 31,528 | 281 | 9.46 |
| 58 | 0 | 9 | Cg1437 | ilvC; ketol-acid reductoisomerase | None | 31 | 36,136 | 338 | 4.55 |
| 59 | 1 | 9 | Cg1790 | pgk; phosphoglycerate kinase | 136 | 37 | 42,671 | 405 | 4.55 |
| 60 | 5 | 9 | Cg2958 | butA; l-2,3-butanediol dehydrogenase/actetoin reductase | 164 | 40 | 27,047 | 258 | 4.62 |
| 61 | 9 | 8 | Cg2211 | Putative membrane protein | 70 | 45 | 16,356 | 147 | 6.36 |
| 62 | 3 | 8 | Cg3227 | lldA; putative l-lactate dehydrogenase | 452 | 32 | 45,686 | 420 | 5.72 |
| 63 | 7 | 8 | Cg1429 | Putative membrane protein | 88 | 42 | 36,855 | 352 | 4.44 |
| 64 | 3 | 8 | Cg1685 | tatX; SEC-independent protein secretion pathway component | 754 | 50 | 12,036 | 105 | 5.28 |
| 65 | 0 | 8 | Cg2444 | Hypothetical protein predicted by Glimmer/Critica | None | 51 | 19,830 | 181 | 4.33 |
| 66 | 8 | 7 | Cg2405 | qcrC; cytochrome c1 | 79 | 30 | 31,134 | 295 | 6 |
| 67 | 8 | 6 | Cg1791 | gap; glyceraldehyde-3-phosphate dehydrogenase | 55 | 43 | 36,023 | 334 | 5.16 |
| 68 | 8 | 6 | Cg1001 | mscL; large conductance mechanosensitive channel | 59 | 44 | 14,526 | 135 | 4.84 |
| 69 | 3 | 7 | Cg1111 | eno; enolase | 144 | 25 | 44,921 | 425 | 4.65 |
| 70 | 1 | 7 | Cg0766 | icd; isocitrate dehydrogenase | 227 | 34 | 80,032 | 738 | 4.72 |
| 71 | 0 | 7 | Cg1290 | metE; homocysteine methyltransferase | None | 36 | 81,263 | 745 | 4.78 |
| 72 | 2 | 7 | Cg1880 | thrS; threonyl-tRNA synthetase | 386 | 54 | 78,124 | 700 | 5.04 |
| 73 | 0 | 6 | Cg2523 | malQ; 4-α-glucanotransferase | None | 39 | 78,475 | 706 | 4.76 |
| 74 | 0 | 6 | Cg2499 | glyS; glycyl-tRNA synthetase (glycine-tRNA ligase) | None | 41 | 53,011 | 461 | 5.28 |
| 75 | 1 | 6 | Cg1737 | acn; aconitase | 449 | 46 | 102,104 | 943 | 4.53 |