Fig. 3.

Macrophage proteins detected in medium and in lysates represent distinct functional categories. Conditioned medium from M9A and WT cells and lysates from M9A cells were analyzed by 2D LC-MS/MS. Proteins were identified and quantified as described in the legend to Fig. 2. Proteins were ranked by the number of peptides identified, and the medium/lysate ratio was calculated. Functional categories were determined using Gene Ontology analysis for lysate proteins (medium/lysate ratio <0.5) (A) or secreted/shed proteins (medium/lysate ratio >1.5) (B). This approach identified multiple distinct categories of proteins that were significantly overrepresented (categories with p < 0.01; plotted as p⁻¹) in secreted/shed proteins and proteins arising from lysis.