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H/D-EX measured by mass spectrometry. Ultrazoom spectra of the Phe227–Trp238 (s1B-s2B) fragment (A), the Thr339–Phe352 (s5A-RSL) fragment (B), and the Met1–Leu30 (N-term-hA) fragment (C) are shown (see “Results”). Dashed lines indicate manually determined average m/z of peptide ions (see “Experimental Procedures”).
