Fig. 4.

Analysis of the Ser hydrolases identified by MudPIT and from protein gels. A, spectral counts in the MudPIT analysis. The S.E. is calculated over all five independent FP labeling experiments. B, proteins that were identified by MudPIT (two-dimensional (2D)) and from 1D protein gel (Fig. 7) are indicated with filled and open circles, respectively. C, identities of the FP-labeled proteins. The Arabidopsis Genome Initiative (AGI) code for each of the identified protein is followed by the common names and a PFAM-predicted structure. D, the identified proteins represent different subclasses in each of the phylogenetic threes. Phylogenetic trees were constructed for each of the subfamilies (see supplemental Fig. S2). The circles indicate the proteins that were identified as FP-labeled proteins: closed, found in MudPIT; bordered, found on 1D gel. SFGH, S-formylglutathione hydrolase; SBT, subtilase; TPP2, tripeptidyl peptidase-2; AARE, acylamino acid releasing enzyme; POPL, prolyl oligopeptidase-like; SCPL, serine carboxypeptidase-like; SNG1, sinapolyglucose accumulator-1; BRS1, Bri1 suppressor-1; PAE, pectineacetylesterase; CXE, carboxyesterase; MES, methylesterase; ACL, acetone cyanohydrin lyase; FSHI, family of serine hydrolases-I.