Fig. 7.

Identities of Ser hydrolases during the Arabidopsis-Botrytis interaction. A, symptoms of Botrytis-infected Arabidopsis leaves at the time of analysis. Leaves of wild-type (Col-0) and pad3 mutant plants were infected with Botrytis using a spore-containing droplet. The picture was taken at 5 dpi. B, profile of purified TriFP-labeled proteins of Botrytis-infected tissues at 5 dpi used for identification. Protein extracts were labeled with TriFP, and biotinylated proteins were purified and separated on a protein gel. 16 fluorescent bands from eight regions (1–8) were excised and digested with trypsin, and eluted peptides were analyzed by LC-MS/MS. C, Ser hydrolases identified from the eight regions. Botrytis proteins are indicated in bold. D, spectral counts of each identified Ser hydrolase from the Col-0 and pad3 samples. E, PFAM structure of each identified Ser hydrolase using the same colors as in Fig. 4. New PFAM structures are cutinases (pink) and lactamases (light gray). For protein abbreviations see legend of Fig. 4C.