Table I.
Abundant proteins evidenced in striatal microdialysate
The table lists proteins for which abundance, as determined by spectrum counting, exceeds 4% of the total amount of protein detected. Microdialysis was performed under basal condition (aCSF perfusion). Proteome analysis was carried out using probes with a 100-kDa (n = 11) or 20-kDa (n = 6) cutoff point. For peptidome analysis (n = 6), we used 20-kDa-cutoff probes. Relative abundance of proteins was deduced from spectrum counting data normalized by molecular mass.
| Description | UniProt ID | Molecular mass | Abundance | Secretory pathway | Molecular function |
|---|---|---|---|---|---|
| Da | % | ||||
| 100-kDa proteome | |||||
| Serum albumin precursor | ALBU_RAT | 68,686 | 20.1 | Classical secretion | Transfer/carrier protein |
| Cystatin-C precursor | CYTC_RAT | 15,427 | 11.0 | Classical secretion | Protease inhibitor activity |
| Apolipoprotein E precursor | APOE_RAT | 35,731 | 8.1 | Classical secretion | Transfer/carrier protein |
| Thymosin β-4 | TYB4_RAT | 5,050 | 6.6 | Non-classical secretion | Structural molecule activity |
| α1-Antiproteinase precursor | A1AT_RAT | 46,107 | 7.0 | Classical secretion | Protease inhibitor activity |
| α1-Inhibitor 3 precursor | A1I3_RAT | 163,670 | 4.9 | Classical secretion | Protease inhibitor activity |
| Proenkephalin A precursor | PENK_RAT | 30,912 | 4.5 | Classical secretion | Neuropeptide hormone activity |
| 20-kDa proteome | |||||
| Neurogranin | NEUG_RAT | 7,492 | 19.8 | Other | Neuropeptide hormone activity |
| Thymosin β-4 | TYB4_RAT | 5,050 | 15.8 | Non-classical secretion | Structural molecule activity |
| Proenkephalin A precursor | PENK_RAT | 30,912 | 13.6 | Classical secretion | Neuropeptide hormone activity |
| Cystatin-C precursor | CYTC_RAT | 15,427 | 13.3 | Classical secretion | Protease inhibitor activity |
| Purkinje cell protein 4 | PCP4_RAT | 107,22 | 9.5 | Non-classical secretion | Calcium-binding protein |
| FK506-binding protein 1A | FKB1A_RAT | 11,915 | 9.5 | Other | Calcium-binding protein |
| β1 globin | Q9QUT6_9MURI | 15,824 | 4.3 | Other | Oxygen transporter activity |
| Peptidome | |||||
| Neurogranin | NEUG_RAT | 7,492 | 42.8 | Other | Neuropeptide hormone activity |
| Fibrinogen α chain precursor | FIBA_RAT | 86,632 | 13.0 | Classical secretion | Extracellular matrix |
| β1 globin | Q9QUT6_9MURI | 15,824 | 12.9 | Other | Oxygen transporter activity |
| Fibrinogen γ chain precursor | FIBG_RAT | 50,600 | 5.7 | Classical secretion | Extracellular matrix |