Fig. 6.

Detection of 20 S core particle in the immunoprecipitated HAUSP complexes. HAUSP complexes were immunoprecipitated using the anti-HAUSP antibody or a control antibody as described under “Experimental Procedures,” separated by SDS-PAGE, and transferred to a nitrocellulose membrane. Rabbit polyclonal antibodies against 20 S core subunits were used for the immunoblot staining. Lane 1, 0.05 μg of commercially available human erythrocyte 20 S proteasome; lanes 2 and 4, whole protein content of eluted proteins from a 1.5-ml erythrocyte aliquot precipitated with the anti-HAUSP antibody (lane 2) or with the OX8 antibody (control) (lane 4); lane 3, protein sample eluted from the anti-HAUSP-Dynabeads after incubation with 1.5 ml of lysis buffer.