Figure 4.
TSAb specific for the human TSHR and the mTSHR. Sera were from nontransgenic mice and Lo-expressor transgenic mice of three Aire genotypes (+/+, +/−, and −/−) tested for TBI and T4 (Figs. 1 and 3). TSAb activity was measured as cAMP generated in CHO cells expressing the human TSHR (A and C) or the mTSHR (B and D). Data are shown as mean + sem. The number of mice in each group was as follows: A, A-subunit Ad immunized nontransgenic mice: Aire +/+ (n = 9), Aire +/− (n = 10), Aire −/− (n = 9); B, Con-Ad immunized nontransgenics: Aire +/+ (n = 3), Aire +/− (n = 4), Aire −/− (n = 4); C, A-subunit Ad immunized Lo-expressor transgenics: Aire +/+ (n = 8), Aire +/− (n = 10), Aire −/− (n = 10). Values in Aire−/− mice were significantly greater than in Aire +/− mice for both human and mouse TSAb: *, P < 0.05 (ANOVA). Values in Aire−/− mice were significantly greater than in Aire +/− mice for mouse TSAb: **, P < 0.05 (ANOVA).