Figure 3.

RT-PCR of chimeric MLL-MSF. RT-PCR with an MLL exon 5 forward primer (EX5NP) and reverse primers MSFR1 (Left) and MSFR5 (Right) in MSF (see Fig. 2). Amplification with EX5NP and MSFR1 yielded a 1,093-bp product in the positive control (plasmid containing the first chimeric PCR product of MLL exon 5 and partial MSF) and in the patient’s cDNA. A product of 1,332 bp was amplified with EX5NP and MSFR5 in the patient’s cDNA. The MSFR5 sequence was in the EST. M, λHindIII digest marker; C, positive control; P, patient’s cDNA; N, negative control (K562 cDNA).