Figure 4.

Northern blot analysis of MSF in normal tissues. Multiple-tissue Northern blots (CLONTECH) were hybridized with a 614-bp PCR product amplified by MSFF2 and MSFR3 (see Fig. 2). A major 4-kb transcript was expressed ubiquitously. An additional 3-kb less abundant transcript was found in spleen, thymus, and peripheral blood leukocytes (PBL). A 1.7-kb transcript also was found in many tissues with a relatively higher level of expression in heart, liver, skeletal muscle, and kidney. Blots were rehybridized with a β-actin probe.