Figure 6.

The ability to form an N/C interaction does not relate to the ability to induce exogenous or endogenous transactivation activity A, Activity of AR variants in transiently transfected PC3 cells with vehicle or 10 nm DHT treatment performed as described in Fig. 2A. B, Activity of AR variants on chromatin integrated ARR3 reporter. PC3-ARR3-C13 cells were transfected with 100 ng AR expression vector and treated, assayed, and immunoblotted as described in A. Data are representative of results also obtained in an independent derived stable transfected clone. C, Interaction of AR variants with DNA in quantitative transient ChIP analysis. ChIP was performed in PC3 cells transfected in 150 mm² plates with AR variants and treated for 1 h vehicle or 10 nm DHT. Data represent the relative enrichment of the FKBP5 locus for vehicle (open bars) and DHT (closed bars) over a nonspecific genomic DNA region from the KLK3 locus by quantitative real-time PCR. Data are representative of three independently performed transient ChIP experiments. Inset shows AR immunoblot of immunoprecipitated material for AR variants. MW, Molecular weight.