Figure 1.

Glucose and LXR activation increase SREBP-1 expression and nuclear localization and lipogenic gene expression. INS-1 cells were cultured for 48 h in media containing 4 or 16.7 mm glucose ± 10 μm T0901317. A, Microsomes and nuclear extracts were fractionated by SDS-PAGE and SREBP-1 immunoreactivity was detected by Western analysis. Results shown are representative of four independent experiments. B, Total RNA was isolated and analyzed for SREBP-1, ACC, FAS, SCD1, SCD2, ABCA1, and ABCG1 mRNA expression by real-time RT-PCR. Control genes cyclophilin and ribosomal protein L32 were unaffected by T0901317 (TO) or glucose (data not shown). Data are relative to cyclophilin and normalized to cells cultured in 4 mm glucose (mean ± sem, n = 4).