Figure 4.

GLUT4 externalization by a membrane targeted active PI 3-kinase is impaired by nelfinavir (Nelf). 3T3-L1 adipocytes were electroporated in the presence of plasmids encoding a membrane-targeted catalytic subunit of PI 3-kinase (p110-CAAX) along with GRP1(PH)-GFP (A) or Myc-GLUT4-GFP (B–D). The cells were treated (when indicated) with 30 μm nelfinavir for 18 h but were not further stimulated with insulin. Shown are representative confocal cell images from three independent experiments for A and eight for B–D. C, Rim score. D, myc/GFP (red/green) fluorescence ratio. *, P < 0.05.