Fig. 1.

Representative Western blots of hypothalamic homogenates are shown for each brain Na⁺-K⁺-ATPase α-isoform and β-tubulin (β-tub). The first 3 lanes of all Western blots were loaded with 1, 2, and 3 μg (α₁ and α₂ blots) or 0.1, 0.2, and 0.3 μg (α₃ blots) of a reference sample. Starting with lane 4, samples were loaded in the order: male wild-type (+/+), male α₂ +/−, female +/+, female α₂ +/−, male +/+, male α₂ +/−, etc. The staggering of group samples was performed to minimize bias due to potential variations from one region of a blot to another. A total of 20 samples from either hypothalamus or pons/medulla were loaded on each blot.