Table 1.

PCR conditions and specific MgCl₂ concentrations for the amplification of PAX2 transcripts and the housekeeping gene PBGD.

PCR conditions
target	MgCl2 (mmol/l)	Cycles	Temperature (°C)	Time (s)
PBGD	4	45	95	0
			65	12
			72	10
PAX2_1	1,5	55	95	0
			55	10
			72	8
PAX2_2	2	55	95	0
			57	12
			72	10