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. 2009 Jun;20(6):1223–1235. doi: 10.1681/ASN.2008050492

Figure 1.

Figure 1.

Loss of tissue inhibitor of matrix metalloproteinases 3 (TIMP3) enhances age-dependent tubulointerstitial fibrosis. (A) Histologic analyses of 2-yr-old TIMP3−/− show enhanced tubulointerstitial injury, shrunken glomerular tufts, and increased fibrosis compared with wild-type (WT) mice. Collagen/volume fraction (Picrosirius red [PSR] staining), collagen type-I, and α-smooth muscle actin (αSMA) quantification are shown for WT (white) and TIMP3−/− kidneys (gray) (n = 5 per genotype; *P < 0.05 compared with WT group). (B) TIMP3 protein levels in young and old mice show a significant reduction in the medullar of the old kidneys (n = 4 per group; *P < 0.05 compared with young group). (C) No protein was detected in the urine of old TIMP3−/− compared with old WT mice. Chemical-grade bovine serum albumin was used as positive control. AU, arbitrary units; PAS, periodic acid-Schiff.