Figure 2.

Comparison and confirmation of the differentially expressed gene by Northern blot analysis and endogenous expression of TBP1 in U87MG vs. U87/T691. (A) B104-1-1 and NR6TintΔ cells were incubated with or without 7.16.4 (10 μg/ml) for 24 h before RNA isolation. Total RNA (10 μg) was loaded in each lane and was probed with human TBP1 cDNA probe. TBP1 expression was up-regulated with anti-p185^neu mAb (7.16.4) treatment in B104-1-1 cells (lanes 1 and 2) but was not changed in internalization defective NR6TintΔ cells (lanes 3 and 4). (B) The corresponding formaldehyde gel electrophoresis of total RNA is shown. (C) The U87/T691 subclone, an epidermal growth factor receptor (EGFR)-positive cell line phenotypically inhibited by the expression of a trans-inhibitory ectodomain form of p185^neu (T691 stop neu), showed increased endogenous levels of TBP1. Relative units are derived from scanning densitometry (Molecular Dynamics).