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Immunostaining for IF1 (red, A) and the ATP5b subunit of the mitochondrial ATPase (green, B). The ratio of the ATP synthase image with respect to the IF1 image (color coded as indicated between values of 0 and 2) (C) reveals that the ratio of ATP5b to IF1 is significantly higher in distal than proximal tubules (D). Results displayed are mean ratio per tubule, from a total of 15 proximal and 18 distal tubules (**P < 0.001). Arrowhead, PT; arrow, DT. Bar = 20 μm.
