TABLE 1.
CYTOKINE mRNA LEVELS IN LUNGS OF MONOCROTALINE-TREATED AND MONOCROTALINE+XNT–TREATED RATS
| Cytokine | Control | MCT | MCT+XNT |
|---|---|---|---|
| TGF-β | 1.23 ± 0.49 (4) | 33.73 ± 2.96* (3) | 6.18 ± 1.68# (4) |
| TNF-α | 0.91 ± 0.16 (7) | 11.46 ± 1.70* (7) | 2.23 ± 0.34# (9) |
| IL-6 | 1.26 ± 0.25 (6) | 55.56 ± 10.25* (6) | 8.88 ± 1.59# (6) |
| IL-1 | 1.01 ± 0.01 (6) | 6.76 ± 1.6* (6) | 2.45 ± 0.38# (6) |
| NF-κB-50 | 0.90 ± 0.15 (8) | 45.72 ± 11.9* (6) | 3.01 ± 0.95# (7) |
| NF-κB-65 | 1.05 ± 0.17 (8) | 4.72 ± 0.51* (8) | 2.10 ± 0.35# (10) |
| MCP-1 | 1.26 ± 0.44 (6) | 32.08 ± 2.63* (6) | 4.22 ± 0.73# (6) |
| IL-10 | 1.42 ± 0.21 (8) | 0.29 ± 0.06* (8) | 3.34 ± 05.86# (10) |
Definition of abbreviations: NF-κB = nuclear factor-κB; MCP = monocyte chemoattractant protein; MCT = monocrotaline; TGF = transforming growth factor; TNF = tumor necrosis factor; XNT = 1-[(2-dimethylamino) ethylamino]-4-(hydroxymethyl)-7-[(4-methylphenyl) sulfonyl oxy]-9H-xanthene-9-one.
Total RNAs from control, MCT- and MCT+XNT–treated rats were isolated and subjected to quantitation by real-time reverse transcription–polymerase chain reaction for cytokines as described. Data are expressed as mean ± SEM. Numbers in the parentheses are the number of experiments. *P < 0.05 vs. control rats, #P < 0.05 versus MCT-treated rats (one-way analysis of variance followed by the Tukey test).