Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Nov 21;40(6):672–682. doi: 10.1165/rcmb.2008-0095OC

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009, American Thoracic Society

PMC Copyright notice

Figure 4. — Failure to reconstitute LTA4H−/− mice with antigen-primed LTA4H+/+ T cells, MNCs, or alveolar macrophages after passive sensitization and challenge. (A) LTA4H−/− mice (recipient mice) received 1 × 10⁷ LTA4H+/+ or LTA4H−/− T cells, or MNC cells intravenously, 2 hours before the first airway challenge with aerosolized OVA. Airway reactivity was assessed by electrical field stimulation of tracheal smooth muscle preparations as described in Materials and Methods. LTA4H+/+ and LTA4H−/− mice receiving no cells are also shown. (B) LTB4 levels in BAL fluid after transfer of T cells or MNC. (C) Passively sensitized LTA4H−/− mice (recipient mice) received 1 × 10⁶ LTA4H+/+ or LTA4H−/− alveolar macrophages intratracheally 24 hours before the first airway challenge with aerosolized OVA as described in Materials and Methods. Airway reactivity was assessed by electrical field stimulation of tracheal smooth muscle preparations. (D) LTB4 levels in BAL fluid after transfer of macrophages. n = 8 in each group. *P < 0.05 comparing passively sensitized and challenged LTA4H+/+ mice to all other groups.

Figure 4. — Failure to reconstitute LTA4H−/− mice with antigen-primed LTA4H+/+ T cells, MNCs, or alveolar macrophages after passive sensitization and challenge. (A) LTA4H−/− mice (recipient mice) received 1 × 10⁷ LTA4H+/+ or LTA4H−/− T cells, or MNC cells intravenously, 2 hours before the first airway challenge with aerosolized OVA. Airway reactivity was assessed by electrical field stimulation of tracheal smooth muscle preparations as described in Materials and Methods. LTA4H+/+ and LTA4H−/− mice receiving no cells are also shown. (B) LTB4 levels in BAL fluid after transfer of T cells or MNC. (C) Passively sensitized LTA4H−/− mice (recipient mice) received 1 × 10⁶ LTA4H+/+ or LTA4H−/− alveolar macrophages intratracheally 24 hours before the first airway challenge with aerosolized OVA as described in Materials and Methods. Airway reactivity was assessed by electrical field stimulation of tracheal smooth muscle preparations. (D) LTB4 levels in BAL fluid after transfer of macrophages. n = 8 in each group. *P < 0.05 comparing passively sensitized and challenged LTA4H+/+ mice to all other groups.