TABLE 2.

EFFECTS OF TOLUENE DIISOCYANATE ON RESPIRATORY PARAMETERS IN Trpa1^+/+ AND Trpa1^−/− MICE

Condition	Genotype	RR (bpm)	Vt (μl)	T Br (ms)
Basal	Trpa1+/+	226 ± 14	231 ± 13	31 ± 7
	Trpa1−/−	227 ± 15	283 ± 28	26 ± 8
Vehicle	Trpa1+/+	227 ± 13	228 ± 19	36 ± 8
	Trpa1−/−	216 ± 7	261 ± 29	60 ± 19
0.1% TDI	Trpa1+/+	194 ± 13	224 ± 25	31 ± 6
	Trpa1−/−	188 ± 16	243 ± 22	46 ± 8
1% TDI	Trpa1+/+	99 ± 15*†	224 ± 25	337 ± 74*†
	Trpa1−/−	190 ± 15	243 ± 22	84 ± 22‡

Definition of abbreviations: RR, respiratory rate; T Br, time of braking; TDI, toluene diisocyanate; Trpa, transient receptor potential ankyrin.

Values are means (±SEM) (n = 7–10) of respiratory parameters (RR, Vt, T Br) measured after acclimatization period (Basal) and delivery of vehicle or irritant.

^*P < 0.01, statistically significant differences between Trpa1^+/+ and Trpa1^−/− (ANOVA followed by Bonferroni post tests).

^†P < 0.01, statistically significant effect of treatment compared to baseline (ANOVA followed by Dunnett's multiple comparison test).

^‡P < 0.05, statistically significant effect of treatment compared to baseline (ANOVA followed by Dunnett's multiple comparison test).