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. 2009 Jun;150(2):584–595. doi: 10.1104/pp.109.136408

Figure 3.

Figure 3.

Bmr6 locus and bmr6 mutation. A, The gene structure of the Bmr6 gene, which is located on chromosome 4. The exons are indicated as solid boxes, and the introns are indicated as lines. B, CAPS marker for Bmr6 locus was designed to verify mutation. PCR primers were used to amplify a 613-bp fragment of Bmr6 from sorghum genomic DNA. The amplification products were digested with the restriction enzyme BsaAI and analyzed by agarose gel electrophoresis. The C-to-T transition mutation in bmr6 created a BsaAI restriction site. The wild-type (WT) DNA sequence is not cleaved by BsaAI, but the bmr6 sequence is, resulting in two fragments of 333 and 280 bp. het, Heterozygote is 1:1 mixture of wild-type and bmr6 genomic DNA. Lane 1, Atlas wild type; lane 2, Atlas bmr6; lane 3, Atlas het; lane 4, R Tx430 wild type; lane 5, R Tx430 bmr6; lane 6, R Tx430 het; lane 7, B Wheatland wild type; lane 8, B Wheatland bmr6; lane 9, B Wheatland het; lane 10, no DNA negative control.