Figure 8.

Predicted protein structure of Bmr6 and SbCAD4 containing the bound substrate. Amino acids 9 to 353 of Bmr6 aligned with the sequence for PtSAD with 51.9% sequence identity. Amino acids 8 to 353 of SbCAD4 sequence aligned with 60.8% identity to PtSAD. The positions of NADP+ and the two zinc ions were taken directly from the PtSAD structure and placed into each structural model. A, The active site of Bmr6 with coniferyl aldehyde bound, including the cofactor with the following depictions: carbon atoms (white), oxygen atoms (red), phosphorus atom (orange), and nitrogen atoms (blue). B, Model of Bmr6 with the active site for SbCAD4 overlayed in the purple. Only the amino acids within 5 Å of the docked substrate are represented.