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Bmr6 and SbCAD4 kinetic parameters for coniferyl alcohol
The enzymatic velocities for Bmr6 and SbCAD4 were measured for a range of concentrations of coniferyl alcohol (Supplemental Fig. S2) to determine the enzyme kinetic parameters (see “Materials and Methods”; n = 4).
| Enzyme Kinetic Parameters | Bmr6 | SbCAD4 |
|---|---|---|
| Km (μm) | 7.5 ± 1.2 | 117 ± 24 |
| Vmax (nmol s−1 mg protein−1) | 140 ± 7 | 2.6 ± 2.6 |
| Vmax\Km (s−1 mg protein−1) | 1.87 × 10−2 | 2.22 × 10−5 |
