Figure 1.

SDS-PAGE of ascorbate-reducible cytochrome b-containing fractions eluted from a Superdex 200 gel filtration chromatography column. A, Histograms representing the relative amount of cytochrome in each fraction (0.4 mL) of the peak (centered at 15.05 mL of elution) of the Superdex 200 column. ABS, Absorbance. B, Fractions were concentrated 12-fold and 15 μL of each concentrated fraction were loaded on the gel. Gel was Coomassie Brilliant Blue stained. Markers are indicated on the left. On the right, black arrowhead indicates the band around 60 kD correlating with the Superdex peak. White arrowhead indicates a contaminating polypeptide. [See online article for color version of this figure.]