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Gel filtration chromatography of purified recAIR12. A, Purified recAIR12 was loaded on the Superdex 200 column. B, The peak of recAIR12 eluted from the gel filtration chromatography in A was concentrated (20-fold), deglycosylated through incubation with EndoH (2.5 μg) overnight at 22°C, and loaded again on the Superdex 200 column. ABS, Absorbance.
