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. 2009 Jun;150(2):658–669. doi: 10.1104/pp.109.136614

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Copyright © 2009, American Society of Plant Biologists

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Figure 4. — Arabidopsis MCM5 and MCM7 proteins are nuclear in G1, S, and G2/M cells. A, Nuclei were isolated from formaldehyde-cross-linked Arabidopsis suspension cells and sorted into G1, S, and G2/M populations based on DNA content by FACS. B, After cross-link reversal, proteins from 200,000 nuclei in G1 (lane 1), S (lane 2), and G2/M (lane 3) phases of the cell cycle were resolved by SDS-PAGE, and the blots were probed with anti-MCM5 (top) and anti-MCM7 (bottom) antibodies. C, Cytoplasmic contamination did not account for the presence of MCM5 and MCM7 in G2/M phase nuclei. Proteins from FACS-sorted G1 (lane 2) and G2/M (lane 4) nuclei and from whole cell extract (WCE; lanes 1 and 3) were resolved by SDS-PAGE. Blots were probed with anti-MCM5 (top) and anti-UGPase (bottom) antibodies.