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. 2009 Jun;150(2):596–605. doi: 10.1104/pp.109.136929

Figure 1.

Figure 1.

Time-dependent inactivation of GALDH with thiol-modifying agents. GALDH (2 μm) was incubated with 50 μm DTNB (A), 50 μm NEM (B), or 5 mm hydrogen peroxide (C) in 50 mm sodium phosphate, pH 7.4, in the presence (white circles) or absence (black circles) of 1 mm l-galactono-1,4-lactone. Data were fitted according to pseudo first-order kinetics. D, Half-life of inactivation of GALDH (2 μm) by hydrogen peroxide (5 mm) as a function of pH. The inset shows the time-dependent inactivation of GALDH (2 μm) by hydrogen peroxide (5 mm) in 50 mm BisTris-Cl, pH 6.0 (black circles), 50 mm Tris-Cl, pH 7.4 (dark-gray circles), 50 mm Tris-Cl, pH 8.0 (light-gray circles), and 50 mm Tris-Cl, pH 8.8 (white circles). For clarity, only selected traces are shown.