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Absorption spectra of thiol-modified GALDH. A, DTNB-treated GALDH (dotted line) and GALDH-TNB treated with GSH (dashed line) compared to untreated GALDH (solid line). B, Hydrogen peroxide-inactivated GALDH (dotted line) and hydrogen peroxide- and GSH-inactivated GALDH (dashed line). Excess reagents were removed by gel filtration prior to recording of the absorption spectra.
