Figure 3.

Deduced amino acid sequences of NnAOX1a and NnAOX1b aligned with those of previously reported AOXs expressed in thermogenic tissues. Bold characters highlight residues conserved across all of the AOX sequences in the alignment. The putative structural features are indicated as follows: asterisks for two highly conserved Cys residues, termed Cys₁ and Cys₂ (Berthold et al., 2000), double underline for ligands to iron atoms of the catalytic center, and gray bars for four α-helices. Abbreviations and data sources are as follows: DvAOX, D. vulgaris AOX (BAD51465); PbAOX, P. bipinnatifidum AOX (BAD51467); SgAOX, S. guttatum AOX (P22185); SrAOX, S. renifolius AOX (BAD83866).