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. 2009 Jun;150(2):844–857. doi: 10.1104/pp.109.137414

Figure 1.

Figure 1.

CCA1 is correctly regulated in CCA1pro∷CCA1-HA-YFP cca1-1#1 and CCA1pro∷CCA1-HA-YFP cca1-1#2 transgenic plants. A, The CCA1-HA-YFP construct used to transformed cca1-1 plants. B, CCA1pro∷CCA1-HA-YFP cca1-1#1, CCA1pro∷CCA1-HA-YFP cca1-1#2, and wild-type plants were grown in LD for 2 weeks before harvested and the levels of CCA1-HA-YFP mRNA and CCA1 mRNA determined by quantitative PCR and plotted on a graph relative to TUB2 mRNA levels. C, CCA1pro∷CCA1-HA-YFP cca1-1#1 and CCA1pro∷CCA1-HA-YFP cca1-1#2 plants were grown in LD for 2 weeks before harvesting, and the levels of CCA1-HA-YFP protein were determined by western analysis. The Coomassie-stained loading control is shown below. The white and black bars represent light and dark periods, respectively. D, One-week-old CCA1pro∷CCA1-HA-YFP cca1-1#1, CCA1pro∷CCA1-HA-YFP cca1-1#2, cca1-1, and wild-type plants were transferred to LL after entrainment in LD. Leaf movements were recorded every 20 min over 7 d and analyzed by FFT-NLLS. The RAE of the rhythms is plotted against period.