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. 2009 Jun;150(2):844–857. doi: 10.1104/pp.109.137414

Figure 4.

Figure 4.

LHY is correctly regulated in LHYpro∷LHY-myc LHY-null transgenic plants. A, The LHY-myc construct used to transform LHY-null plants. B, LHYpro∷LHY-myc LHY-null and wild-type plants were grown in LD for 2 weeks before being harvested, and the levels of LHY-myc mRNA and LHY mRNA were determined by quantitative PCR and plotted on a graph relative to TUB2 mRNA levels. C, LHYpro∷LHY-myc LHY-null plants were grown in LD for 2 weeks before being harvested, and the levels of LHY-myc protein were determined by western analysis. The Coomassie-stained loading control is shown below. The white and black bars represent light and dark periods, respectively. D, One-week-old LHYpro∷LHY-myc LHY-null, LHY-null, and wild-type plants were transferred to LL. Leaf movements were recorded every 20 min over 7 d and analyzed by FFT-NLLS. The RAE for the rhythms was plotted against the period.