Table I.
Primer and probe sequences used in this study
| Name of Primer or Probe | Sequence |
|---|---|
| TQM tefA fwd | TCTACCACCACCGGTCACTTGATT |
| TQM tefA rev | TGAGCTTGTCAAGAACCCACGCAT |
| TQM tefA Probe | TET-TGCGGTGGAATTGACAAGCGTACCAT--BHQ_1 |
| TQM lolF2 fwd | CTACAGACTGCGAAAGCTCGGATT |
| TQM lolF2 rev | GGAGAAGCTCTGCATCATAGAACTGG |
| TQM lolF2 Probe | 6-FAM--CTTATCCGGGAGCAGCAGTCGACAG--BHQ_1 |
| TQM lolD2 fwd | TCTTTGTTGCCGACTTGAACGACG |
| TQM lolD2 rev | ATGCCCAAGGACAGGATCAACTCA |
| TQM lolD2 Probe | 6-FAM--AAAGCAGCTATGATCGACGGCTGATCCA--BHQ_1 |
| TQM lolO2 Fwd | TTCTTGCACCAGACGAATGCTTCC |
| TQM lolO2 rev | AATACTTGCGACAGCTTGACGAGG |
| TQM lolO2 Probe | 6-FAM-ATAACATAGACGGCTCCGTGATGGCT-BHQ_1 |
| TQM lolA2 fwd | TCGCCACCATGGATGCCAATGATA |
| TQM lolA2 rev | TTTAGCAGTGTGCTGCTCCGAGAT |
| TQM lolA2 Probe | 6-FAM-TTCTCACGGTCATGATTTCGCACGAC-BHQ_1 |
| TQM lolU2 fwd | ATGACAACGACGTTCAAGCCTCCT |
| TQM lolU2 rev | ACTTTCTGGCTTCCGTCATGGAGA |
| TQM lolU2 Probe | 6-FAM-AACTCCTGGAGAAGACTTTCGCGCA-BHQ_1 |
| TQM lolP1 fwd | ACCTGTCGACTTCTCTCGTCTGAT |
| TQM lolP1 rev | AGGTAGGTCAGCATCTTGTCAACG |
| TQM lolP1 Probe | 6-FAM-AAGACGGAGACGTGTTCGGCTACGT-BHQ_1 |
| TQM lolT2 fwd | TAGCCACTTGTGGCAATCAGAGAC |
| TQM lolT2 rev | GCGTATGCCAGAAGGAATGCATCA |
| TQM lolT2 Probe | 6-FAM-TGCAGCTCCTGGAGATTGACCTCAAA-BHQ_1 |
| TQM lolE2 fwd | TGGAGCCTAACAAGACGGACCAAA |
| TQM lolE2 rev | TGAGCCGGTGGGCGTAGAATTTAT |
| TQM lolE2 Probe | 6-FAM-AGCCGTCTTTGGCACCTACCACTTT-BHQ_1 |
| TQM C2H2 TF fwd | AGCCTGGCCATACGTTTCGATGTA |
| TQM C2H2 TF rev | AGCCCAGGAAATCACAAGGGTAGA |
| TQM C2H2 TF Probe | 6-FAM-ACAAGAGCTATTCTCGCGCAGGACAT-BHQ_1 |
| ProC Efes fwd | GCATGGCGATTTCACCATGACG |
| ProC Efes rev | CATCCAGTGGTGTTGAATCTGG |
| TQM ProC fwd | CGTCCATGTTGTTCAATGGCAGGT |
| TQM ProC rev | AACACTGCTGCTCTCATACGGGAA |
| TQM ProC Probe | 6-FAM-TATCCAGGTAACCAGGGCCTTGGATT-BHQ_1 |
| MetE EFes fwd | AGCCCAGTAACTGCGAAAGATGGA |
| MetE Efes rev | ATCATGGCCCTCAGGACTGTCAAT |
| TQM MetE fwd | ACTTGGAGGCATGTTCGTTCTGGA |
| TQM MetE rev | ATGCTTTGTCGTTGTGCTTCACCC |
| TQM MetE Probe | 6-FAM-ATACGATGCATCGTTCCCATTGCCACGT-BHQ_1 |
| TQM lolF1 fwd | CAATCTTGGAGAAGCTCCGCATCA |
| TQM lolF1 rev | CTCGCTGTCTACAATGACTACAGA |
| TQM lolF1 Probe | 6-FAM-AGAAGGGAAACAGGCTGTCGACTGCT-BHQ_1 |
| TQM lolE1 fwd | TGGATCCTAACAAGACGGACCAAA |
| TQM lolE1 rev | AGCCGGTGGGCGTAGAATTTG |
| TQM lolE1 probe | 6-FAM-AGCTGTCTTTGGCACCTACCACTTTGA-BHQ_1 |
| TQM lolA1 fwd | CCACCATGGATGCCAATGATATTCC |
| TQM lolA1 rev | TTTAGCAGTGTACTGCTCCGAGAT |
| TQM lolA1 probe | 6-FAM-TTCCCACGGTCATGATTTCGCACGAC-BHQ_1 |
| TQM lolU1 fwd | GTGACAACAACGTTCAAGATTCCTTCG |
| TQM lolU1 rev | ACTTTCTGGCTCCCGTCATGGA |
| TQM lolU1 probe | 6-FAM-AGCTCCTGGAGAAGACTGTCGCGCA-BHQ_1 |
| TQM lolO1 fwd | TTCTTGCACCAGGCGAATGCTTC |
| TQM lolO1 rev | AATACTTGCGACAGCTCGACGAGG |
| TQM lolO1 probe | 6-FAM-ATAACGTAGATGGCTCCGTGATGGCTC-BHG_1 |
| TQM lolD1 fwd | CCGACTTGAACGACATTGTTCGTAAGT |
| TQM lolD1 rev | ATGCCCAAGGAGAGGATCAACTCAA |
| TQM lolD1 probe | 6-FAM-AAAGCAGCTACGATCGACGGCTGATCCA-BHQ_1 |
| TQM lolT1 fwd | TAACCACTTGTGGCAATCAGAGAC |
| TQM lolT1 rev | GTATGCCAGAAGGAAGGCATCA |
| TQM lolT1 probe | 6-FAM-TGCAGCTCCTGGAGATTGACCTCGAA-BHQ_1 |
| Lolium EF1-α fwd | TTGAGAGGTCCACCAACCTTGACT |
| Lolium EF1-α rev | GCACAGTTCCAATGCCACCAATCT |
| Lolium EF1-α probe | 6-FAM-TTGAGGCTCTTGACCAGATCAATGAGC -IABLFQ |