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RpoSK173E is not subject to proteolysis. Cells expressing RpoSwt (●) or RpoSK173E (▴) from pBAD18 were grown in minimal medium M9 with 0.4% glycerol. RpoS degradation was demonstrated by pulse–chase labeling and immunoprecipitation of exponential phase samples (harvested at an OD578 of 0.6) as detailed in Materials and Methods.
