Figure 6.

The K173E mutation in RpoS differentially affects the expression of various RpoS-dependent genes. Strains carrying rssB∷cat, rpoS∷Tn10, and lacZ fusions in the RpoS-controlled genes osmY, bolA, csiD, and otsB, which express different RpoS variants from pBAD18-derived plasmids, were grown in LB. Two hours after entry into stationary phase, specific β-galactosidase activities were determined. In each panel representing one of the reporter gene fusions, the bars represent strains carrying the following RpoS variants (from left to right): RpoS^wt, RpoS^K173E, RpoS^E174T, RpoS^V177K, and RpoS^Y178L. Values given are averages of two independent cultures, each of which was sampled in triplicate.