Fig. 1.

Distribution of endogenous calneurons compared with NCS-1 and caldendrin. (A) Note that in hippocampal primary neurons calneurons are much more restricted to the Golgi and show a much better overlap with PI-4Kβ than caldendrin and NCS-1. (Scale bar: 20 μm.) (B) Coimmunoprecipitation of calneuron-1 and calneuron-2 with PI-4Kβ from a rat brain extract. Rabbit polyclonal calneuron antibodies were used for immunoprecipitation, and the immunoblots were processed with a mouse PI-4Kβ antibody. Similar amounts of a rabbit IgG served as a control. Unb, unbound; IP, immunoprecipitate; rb, rabbit.