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. 2009 May 18;106(22):9010–9015. doi: 10.1073/pnas.0901329106

Fig. 4.

Fig. 4.

Expression of activation markers and apoptosis of T cells stimulated with anti-CD3/CD28 antibodies and IFN-α or IFN-γ in breast cancer patients and healthy controls. Lymphocytes from BC patients (filled circles) and healthy controls (open squares) were stimulated with anti-CD3 and anti-CD28 antibody-coated beads alone or with IFN-α or -γ, or unstimulated. The % T cells positive for CD25, HLA-DR, CD54, CD95, Annexin V (Anx), and ViViD (Viv) were measured by FACS at 48 h. (A) % CD25+; (B) % HLA-DR+; (C) % CD54+; (D) % CD95+; (E) IFN-induced change in % CD95+ activated T cells (% CD95+ in conditions stimulated with anti-CD3 anti-CD28 plus IFN-α or -γ minus the % CD95+ in conditions stimulated only with anti-CD3 anti-CD28); (F) % Anx+ Viv+ (all T cells that were positive for either or both Anx and Viv). The median is indicated by the bar in each data set. Two-sided Wilcoxon-Mann-Whitney tests were used to compare data from breast cancer patients and healthy controls; P-values ≤0.05 are denoted; #1 P = 0.004, #2, P = 0.020, #3 P = 0.018, #4 P = 0.016, #5 P = 0.018, #6 P = 0.053. Multivariate analyses are shown in Table S3.