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. Author manuscript; available in PMC: 2009 Aug 8.
Published in final edited form as: Mol Cell. 2008 Aug 8;31(3):415–421. doi: 10.1016/j.molcel.2008.06.008

Figure 3. The MALT1-activated caspase-8 cannot generate the apoptotic form of caspase-3 but is capable of cleaving c-FLIPL.

Figure 3

(A) [35S]Caspase-3 was incubated with Fv-Caspase-8 alone or Fv-Caspase-8 plus FRB-MALT1 with or without dimerizer.

(B) [35S]Fv-FLIP was incubated with Fv-C8 and Fv-MALT1 as indicated. The reaction mixes were analyzed by autoradiography (left) and anti-caspase-8 immunoblotting (right). *, the large subunit of FLIP that is generated in vitro. **, endogenous caspase-8 in the reticulocyte lysates.

(C) [35S]Fv-FLIP was co-incubated with the indicated Fv-C8 and FRB-MALT1 proteins. The reaction mixes were analyzed as in (A).

(D) CD4+ T cells were stimulated with α-hCD3/hCD28. c-FLIP proteins and actin were detected by Western blot.

(E) Human CD4+ T cells were transfected with control or c-FLIP siRNA plus m/hCD28. Cells were stimulated with α-hCD3/mCD28 beads. IL-2 induction was measured by quantitative RT-PCR.