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Design of a triblock copolymer for promotion of endosome escape. (a) The triblock copolymer PEG–PMPA–PLys system performs specific functions; the PEG segment improves biocompatibility, the PMPA segment contains inherent buffering capacity to promote early endosome escape and the PLys segment facilitates packaging pDNA in the micelle core. (b) Schematic of the hypothesized three-layered micelle formed from the triblock copolymer and pDNA with a spatially regulated structure.
