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. 2009 Mar 25;6(Suppl 3):S293–S309. doi: 10.1098/rsif.2008.0499.focus

Figure 2.

Figure 2

Concept of temperature-responsive chromatography: (a) hydration and dehydration of grafted polymer under an external temperature change ((i) hydrated, expanded PIPAAm and (ii) dehydrated, shrunken PIPAAm), (b) hydrophobic interaction with analyte and (c) chromatograms ((i) 5°C and (ii) 35°C) of steroid analytes separated on PIPAAm-grafted silica beads. Peaks: 1, benzene; 2, hydrocortisone; 3, prednisolone; 4, dexamethasone; 5, hydrocortisone acetate; 6, testosterone. Adapted from Kanazawa et al. (1996). Copyright 1996, American Chemical Society.