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Concept of cell sheet engineering. (a) Recovered cell sheet from a temperature-responsive TCPS dish retains the extracellular matrix (ECM) beneath the cell sheet and cell–cell junctions. (b(i)) Single cell sheet useful for skin or cornea transplantation, (ii) layered cell sheets used to reconstruct homogeneous three-dimensional tissues, including myocardium, and (iii) heterotypic layered cell sheets used to fabricate laminar structures, including the liver and kidneys.
