Figure 7.

Harvested oral mucosal epithelial cell sheets. Primary canine oral epithelial cells were cultured on (a, b) temperature-responsive culture dishes and (c–e) temperature-responsive culture inserts under the various conditions (a) with or (b–e) without 3T3 feeder layers, in the presence of (a–c) FBS or (d) autologous serum or (e) in the absence of serum. On temperature-responsive culture dishes, square temperature-responsive domains were surrounded by non-cell adhesive area, while all culture insert surfaces were completely grafted with temperature-responsive polymer. (a–e) Macroscopic views and (f–j) haematoxylin and eosin-stained paraffin sections. Adapted from Murakami et al. (2006a).