Figure 1.

Predicted protein sequence of PfSUB2 (GenBank accession no. AJ132006) and the alignment of its predicted catalytic domain with B. amyloliquefaciens BPN subtilisin (GenBank accession no. X00165). The translation initiation and stop codons are in bold and underlined. PfSUB2 protein sequence begins with a signal peptide shown in bold. The four residues that compose the subtilase active site are heavily overlined, and the C-terminal transmembrane segment is double underlined. The interruption of the ORF by an intron is shown by ↕. Brackets define the 259 residues of the catalytic domain used to perform the phylogenetic analysis, and the sequence corresponding to the GST-fusion protein is presented in italics and dashed underlined. The two proposed activation sites are underlined.