Figure 4.

Analysis of Pfsub2 mRNA expression through the P. falciparum asexual cycle. RT-PCR was performed on total RNA isolated between 6 and 46 hr after invasion of erythrocytes by merozoites of P. falciparum Palo Alto strain cultivated in vitro. (A) Pfsub2 oligonucleotides have been chosen to flank the Pfsub2 intron. The expected sizes of the amplified fragments are 880 bp and 737 bp on genomic DNA and cDNA, respectively. (B) RT-PCR was performed by using two Pfrab6 oligonucleotides, where one oligonucleotide crossed the junction of the two first exons of Pfrab6 and therefore only cDNA was amplified. As negative controls RT-PCR on water were performed.