Figure 5.

Western blot analysis on protein extracts prepared from P. falciparum segmented schizonts and merozoites. Lanes 1 and 2 correspond to Na₂CO₃-soluble and Na₂CO₃-insoluble protein extracts prepared from segmented schizonts. Lanes 1′ and 2′ contain the same proportion of Na₂CO₃-soluble and –insoluble protein extracts prepared from noninfected RBCs. Lanes 3 and 3′ and lanes 4 and 4′ show Western blot analysis performed on Na₂CO₃-soluble and -insoluble merozoite protein extracts. Lanes 1 and 2, lanes 1′ and 2′, and lanes 3 and 4 have been incubated with anti-GST-PfSUB2 antibodies, and lanes 3′ and 4′ have been incubated with antibodies to GST alone. Lanes 5 and 6 correspond to the immunoprecipitation of segmented schizont S³⁵-labeled proteins performed with mouse anti-GST-PfSUB2 and anti-GST.