Figure 6.

Localization of PfSUB2 protein by immunofluorescence on segmented schizonts and by IEM on fixed merozoites. (A and B) An air-dried fixed segmented schizont incubated with 25 μg/ml of propidium iodide to label the parasite nuclei (A) and mouse anti-GST-PfSUB2 antibodies (B). Bound antibodies then were detected with FITC-coupled anti-mouse antibodies (magnification: ×1,500). (C) The IEM analysis of thin sections of resin-embedded merozoites probed with mouse anti-GST-PfSUB2 antibodies and revealed with gold-labeled anti-mouse IgG antibody. M, mitochondrion.