Abstract
Malassezia furfur, a recently described agent of catheter-associated sepsis, requires long-chained fatty acids for in vitro growth. To determine optimum conditions for recovery of the organism from blood culture, we compared a radiometric (BACTEC) with a lysis centrifugation-direct agar plating (Isolator) blood culture method. When blood culture isolates of M. furfur were suspended in phosphate-buffered saline and used as inocula, the BACTEC system detected the organisms radiometrically only when bottles were supplemented with lipid compounds; detection was often delayed (greater than or equal to 72 h) when small inoculum sizes were tested. The Isolator pediatric tube system detected growth of M. furfur within 48 h of plating onto a lipid-supplemented agar medium. Simulated blood culture experiments showed that the addition of whole human blood and Intralipid to the BACTEC 6B bottle was associated with rapid lysis of erythrocytes, accumulation of a chocolate brown sediment in the bottles, and fungicidal activity to the M. furfur isolates. In contrast, suspensions of M. furfur in whole human blood were stable for at least 8 h in Isolator tubes and quantitatively recoverable after plating onto agar. Of the two systems studied, the Isolator tube system provided a more suitable procedure for isolation of M. furfur from blood culture.
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- Adeniyi-Jones C. C., Stevens D. L., Rasquinha E. S. False no-growth blood cultures in pneumococcal pneumonia. J Clin Microbiol. 1980 Oct;12(4):572–575. doi: 10.1128/jcm.12.4.572-575.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bille J., Edson R. S., Roberts G. D. Clinical evaluation of the lysis-centrifugation blood culture system for the detection of fungemia and comparison with a conventional biphasic broth blood culture system. J Clin Microbiol. 1984 Feb;19(2):126–128. doi: 10.1128/jcm.19.2.126-128.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bille J., Stockman L., Roberts G. D., Horstmeier C. D., Ilstrup D. M. Evaluation of a lysis-centrifugation system for recovery of yeasts and filamentous fungi from blood. J Clin Microbiol. 1983 Sep;18(3):469–471. doi: 10.1128/jcm.18.3.469-471.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Faergemann J., Bernander S. Micro-aerophilic and anaerobic growth of Pityrosporum species. Sabouraudia. 1981 Jun;19(2):117–121. [PubMed] [Google Scholar]
- Faergemann J. Quantitative culture of Pityrosporon orbiculare. Int J Dermatol. 1984 Jun;23(5):330–333. doi: 10.1111/j.1365-4362.1984.tb04062.x. [DOI] [PubMed] [Google Scholar]
- Fischer G. W., Longfield R., Hemming V. G., Valdes-Dapena A., Smith L. P. Pneumococcal sepsis with false-negative blood cultures. Am J Clin Pathol. 1982 Sep;78(3):348–350. doi: 10.1093/ajcp/78.3.348. [DOI] [PubMed] [Google Scholar]
- Hassall E., Ulich T., Ament M. E. Pulmonary embolus and Malassezia pulmonary infection related to urokinase therapy. J Pediatr. 1983 May;102(5):722–725. doi: 10.1016/s0022-3476(83)80244-3. [DOI] [PubMed] [Google Scholar]
- Long J. G., Keyserling H. L. Catheter-related infection in infants due to an unusual lipophilic yeast--Malassezia furfur. Pediatrics. 1985 Dec;76(6):896–900. [PubMed] [Google Scholar]
- Porro M. N., Passi S., Caprill F., Nazzaro P., Morpurgo G. Growth requirements and lipid metabolism of Pityrosporum orbiculare. J Invest Dermatol. 1976 Mar;66(3):178–182. doi: 10.1111/1523-1747.ep12481919. [DOI] [PubMed] [Google Scholar]
- Powell D. A., Aungst J., Snedden S., Hansen N., Brady M. Broviac catheter-related Malassezia furfur sepsis in five infants receiving intravenous fat emulsions. J Pediatr. 1984 Dec;105(6):987–990. doi: 10.1016/s0022-3476(84)80096-7. [DOI] [PubMed] [Google Scholar]
- Prober C. G., Ein S. H. Systemic tinea versicolor, or how far can furfur go? Pediatr Infect Dis. 1984 Nov-Dec;3(6):592–592. doi: 10.1097/00006454-198411000-00022. [DOI] [PubMed] [Google Scholar]
- Redline R. W., Dahms B. B. Malassezia pulmonary vasculitis in an infant on long-term Intralipid therapy. N Engl J Med. 1981 Dec 3;305(23):1395–1398. doi: 10.1056/NEJM198112033052307. [DOI] [PubMed] [Google Scholar]
- Redline R. W., Redline S. S., Boxerbaum B., Dahms B. B. Systemic Malassezia furfur infections in patients receiving intralipid therapy. Hum Pathol. 1985 Aug;16(8):815–822. doi: 10.1016/s0046-8177(85)80253-7. [DOI] [PubMed] [Google Scholar]