Figure 4.

Identification of YplA as a 35.5-kDa secreted protein. Extracellular proteins were concentrated from culture supernatants of strains YEDS10/pGY20, GY460/pGY20, and GY460/pVLT33 grown in T medium with 50 μM IPTG to induced transcription from the ptac promoter. Proteins were separated on 12.5% SDS-polyacrylamide gel and stained with silver. Lanes: 1, GY460/pGY20; 2, YEDS10/pGY20; 3, GY460/pGY20; and 4, GY460/pVLT33. Each lane contains the equivalent of 1.5 ml culture at an OD₆₀₀ of 1.0. The position of YplA is marked with an open arrow, and other Fops are marked with solid arrowheads. The location of flagellin is indicated with a bracket.