Immunostaining of representative samples from unwounded human gingival tissue (day 0) and a 7-day-old human gingival wound. Higher staining intensity of αv, β6, pan transforming growth factor (TGF)-β (TGF-β1, 2, 3), TGF-β1, TGF-β3 (A), and latent TGF-β binding protein 1 (LTBP-1), TSP-1, matrix metalloproteinase (MMP)-9, connective tissue growth factor (CTGF), and pro-collagen-I (B) was found in the 7-day-old human gingival wound basal epithelium compared with unwounded gingiva. EP, epithelium; CT, connective tissue; GT, granulation tissue. Bar = 200 μm.