Figure 6.
Inhibition of steroidogenic enzyme mRNA levels by IsoQ compound nos. 31 and 32, and IsoQ A. CYP21A2 and CYP17A1 transcript levels were measured by RT-qPCR in H295R TR/SF-1 cells kept for 4 d in basal conditions or treated with Dox (1 μg/ml) in the absence or presence of the no. 31 (pale gray), no. 32 (dark gray), or IsoQ A (black) compounds (10−5 m each) (mean ± sem of four experiments). *, Values significantly different from the corresponding untreated condition (P < 0.01, two way-ANOVA with Bonferroni posttest).